A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia

Summary Rotylenchus bunae n. sp. was discovered in the coffee rhizosphere Gera district of Jimma, Ethiopia, and has been morphologically molecularly characterised. The new species is identified by a female body length 725-876 μ m, hemispherical lip region with 4-5 annuli, stylet 28-30 lateral field areolation only pharyngeal region, no cuticular striations, gland overlapping intestine dorsally 15-26 double epiptygma, clear spermathecae, vulva position at 54-58% from anterior end, prominent fasciculi mid posterior body, rounded or sometimes slightly truncated, coarsely crenated tail 11-13 phasmids located 7-16 annuli to anus. No males were detected. This found closely related other African species, such as R. unisexus wimbii ; however, it could be well separated them both morphology molecular data (D2-D3 28S partial 18S rDNA).